Isolation and purification of cardiolipin from beef heart.

Novel preparation of cardiolipin from beef heart.

A new method is described for the isolation of beef heart cardiolipin. A lipid-protein complex, rich in cardiolipin, is obtained by a one-step solvent fractionation of the tissue total lipid extract. Cardiolipin in the complex is largely freed of protein by salt denaturation and is further purified by gel filtration on Sephadex LH-20 followed by column chromatography on bicarbonate-treated sili...

Acid Cardiolipin and an Improved Method for the Preparation of Cardiolipin from Beef Heart

In a previous report (1) the isolation of a new phospholipid from beef heart was described. The name “cardiolipin” was suggested for this substance, which is of particular interest in connection with serodiagnostic tests for syphilis (2). The original method of preparation was tedious and involved considerable losses. An improved and simplified method is here described. The development of the n...

Isolation and Purification of a Serologically Active Phospholipid from Beef Heart

Isolation and purification of mitochondrial carnitine octanoyltransferase activities from beef heart.

The purpose of this study was to characterize the mitochondrial carnitine octanoyltransferase of beef heart. Carnitine acyltransferase activities were solubilized from isolated beef heart mitochondria using KC1 and the nonionic detergent, Triton X-100, at final concentrations of 1 M and 2%, respectively. Upon fractionation of the solubilized protein on Cibacron Blue Sepharose, two protein peaks...

Activation of beef-heart cytochrome c oxidase by cardiolipin and analogues of cardiolipin.

Beef-heart cytochrome c oxidase lacking endogenous lipids can be prepared by cholate-mediated exchange with dimyristoylphosphatidylcholine (Powell, G. L., Knowles, P. F. and Marsh, D. (1985) Biochim. Biophys. Acta 816, 191-194). These preparations retained practically no endogenous cardiolipin (less than 0.19 mol cardiolipin per mol of oxidase) but in Tween 80 they retained unaltered electron t...